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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
30/10/2002 |
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Data da última atualização: |
30/10/2002 |
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Autoria: |
McEVORY, T. G.; COULL, G. D.; BROADBENT, P. J.; HUTCHINSON, J. S. M.; SPEAKE, B. K. |
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Título: |
Fatty acid composition of lipids in immature cattle, pig and sheep oocytes with intact zona pellucida. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Journal of Reproduction and Fertility, v. 118, n. 1, p. 163-170, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
Cattle, pig and sheep oocytes isolated from healthy cumulus-oocyte complexes were pooled, within species, to provide samples of immature denuded oocytes with intact zona pellucida (n = 1000 per sample) for determination of fatty acid mass and composition in total lipid, constituent phospholipid and triglyceride. Acyl-containing' lipid extracts, transmethylated in the presence of a reference penta-decaenoic acid (15:0), yielded fatty acid methyl esters which were analysed by gas chromatography. Mean (:t SEM) fatty acid content in samples of pig oocytes (161 +- 18 ug per 1000 oocytes) was greater than that in cattle (63 +- 6 ug; P < 0.01) and sheep oocytes (89 +- t 7 ug; P < 0.05). Of 24 fatty acids detected, palmitic (16:0; 25-35%, w/w), stearic (18:0; 14-16%) and oleic (18:1n-9; 22-26%) acids were most prominent in alI three species. Saturated fatty acids (mean = 45-55%, w/w) were more abundant than mono- (27-34%) or polyunsaturates (11-21%). Fatty acids of the n-6 series, notably linoleic (18:2n-6; 5-8%, w/w) and arachidonic acid (20:4n-6; 1-3%), were the most abundant polyunsaturates. Phospholipid consistently accounted for a quarter of alI fatty acids in the three species, but ruminant oocytes had a lower complement of polyunsaturates (14-19%, w/w) in this fraction than pig oocytes (34%, w/w) which, for example, had a three- to fourfold greater linoleic acid content. An estimated 74 ng of fatty acid was sequestered in the triglyceride fraction of individual pig oocytes compared with 23-25 ng in ruminant ) oocytes (P < 0.01). It is concluded that the greater fatty acid content of pig oocytes is primarily due to more abundant triglyceride reserves. Furthermore, this species- specific difference, and that in respect of polyunsaturated fatty acid reserves, mar underlie the contrasting chilling, culture and cryopreservation sensitivities of embryos derived from pig and ruminant (cattle, sheep) oocytes. MenosCattle, pig and sheep oocytes isolated from healthy cumulus-oocyte complexes were pooled, within species, to provide samples of immature denuded oocytes with intact zona pellucida (n = 1000 per sample) for determination of fatty acid mass and composition in total lipid, constituent phospholipid and triglyceride. Acyl-containing' lipid extracts, transmethylated in the presence of a reference penta-decaenoic acid (15:0), yielded fatty acid methyl esters which were analysed by gas chromatography. Mean (:t SEM) fatty acid content in samples of pig oocytes (161 +- 18 ug per 1000 oocytes) was greater than that in cattle (63 +- 6 ug; P < 0.01) and sheep oocytes (89 +- t 7 ug; P < 0.05). Of 24 fatty acids detected, palmitic (16:0; 25-35%, w/w), stearic (18:0; 14-16%) and oleic (18:1n-9; 22-26%) acids were most prominent in alI three species. Saturated fatty acids (mean = 45-55%, w/w) were more abundant than mono- (27-34%) or polyunsaturates (11-21%). Fatty acids of the n-6 series, notably linoleic (18:2n-6; 5-8%, w/w) and arachidonic acid (20:4n-6; 1-3%), were the most abundant polyunsaturates. Phospholipid consistently accounted for a quarter of alI fatty acids in the three species, but ruminant oocytes had a lower complement of polyunsaturates (14-19%, w/w) in this fraction than pig oocytes (34%, w/w) which, for example, had a three- to fourfold greater linoleic acid content. An estimated 74 ng of fatty acid was sequestered in the triglyceride fraction of individual pig oocytes co... Mostrar Tudo |
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Palavras-Chave: |
Lipídios; Oócitos; Ovocito. |
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Thesagro: |
Bovino; Ovino; Reprodução Animal; Suíno. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02644naa a2200253 a 4500
001 1528781
005 2002-10-30
008 2000 bl uuuu u00u1 u #d
100 1 $aMcEVORY, T. G.
245 $aFatty acid composition of lipids in immature cattle, pig and sheep oocytes with intact zona pellucida.
260 $c2000
520 $aCattle, pig and sheep oocytes isolated from healthy cumulus-oocyte complexes were pooled, within species, to provide samples of immature denuded oocytes with intact zona pellucida (n = 1000 per sample) for determination of fatty acid mass and composition in total lipid, constituent phospholipid and triglyceride. Acyl-containing' lipid extracts, transmethylated in the presence of a reference penta-decaenoic acid (15:0), yielded fatty acid methyl esters which were analysed by gas chromatography. Mean (:t SEM) fatty acid content in samples of pig oocytes (161 +- 18 ug per 1000 oocytes) was greater than that in cattle (63 +- 6 ug; P < 0.01) and sheep oocytes (89 +- t 7 ug; P < 0.05). Of 24 fatty acids detected, palmitic (16:0; 25-35%, w/w), stearic (18:0; 14-16%) and oleic (18:1n-9; 22-26%) acids were most prominent in alI three species. Saturated fatty acids (mean = 45-55%, w/w) were more abundant than mono- (27-34%) or polyunsaturates (11-21%). Fatty acids of the n-6 series, notably linoleic (18:2n-6; 5-8%, w/w) and arachidonic acid (20:4n-6; 1-3%), were the most abundant polyunsaturates. Phospholipid consistently accounted for a quarter of alI fatty acids in the three species, but ruminant oocytes had a lower complement of polyunsaturates (14-19%, w/w) in this fraction than pig oocytes (34%, w/w) which, for example, had a three- to fourfold greater linoleic acid content. An estimated 74 ng of fatty acid was sequestered in the triglyceride fraction of individual pig oocytes compared with 23-25 ng in ruminant ) oocytes (P < 0.01). It is concluded that the greater fatty acid content of pig oocytes is primarily due to more abundant triglyceride reserves. Furthermore, this species- specific difference, and that in respect of polyunsaturated fatty acid reserves, mar underlie the contrasting chilling, culture and cryopreservation sensitivities of embryos derived from pig and ruminant (cattle, sheep) oocytes.
650 $aBovino
650 $aOvino
650 $aReprodução Animal
650 $aSuíno
653 $aLipídios
653 $aOócitos
653 $aOvocito
700 1 $aCOULL, G. D.
700 1 $aBROADBENT, P. J.
700 1 $aHUTCHINSON, J. S. M.
700 1 $aSPEAKE, B. K.
773 $tJournal of Reproduction and Fertility$gv. 118, n. 1, p. 163-170, 2000.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio-Norte. Para informações adicionais entre em contato com cpamn.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
18/11/1993 |
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Data da última atualização: |
09/03/2000 |
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Autoria: |
EMBRAPA (Brasilia, DF). |
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Título: |
Sistemas de producao para a cultura do arroz. |
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Ano de publicação: |
1975 |
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Fonte/Imprenta: |
Pelotas: EMBRAPA, 1975. |
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Páginas: |
25p. |
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Série: |
(EMBRAPA. Sistemas de producao. Circular, 36). |
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Idioma: |
Português |
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Conteúdo: |
Arroz: Construcao e conservacao de ruas para colheita a granel; Construcao e conservacao dos sistemas de drenagem; Preparo do solo; Adubacao, semeadura e cultivares; Entaipamento; Irrigacao; Controle as invasoras; Controle as pragas e molestias; Colheita; Secagem. |
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Palavras-Chave: |
Brasil; Cultural methods; Production; Rio Grande do Sul; Sistema. |
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Thesagro: |
Arroz; Oryza Sativa; Pratica Cultural; Produção; Sistema de Produção. |
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Thesaurus NAL: |
rice. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00938nam a2200265 a 4500
001 1057245
005 2000-03-09
008 1975 bl uuuu u0uu1 u #d
100 1 $aEMBRAPA (Brasilia, DF)
245 $aSistemas de producao para a cultura do arroz.
260 $aPelotas: EMBRAPA$c1975
300 $a25p.
490 $a(EMBRAPA. Sistemas de producao. Circular, 36).
520 $aArroz: Construcao e conservacao de ruas para colheita a granel; Construcao e conservacao dos sistemas de drenagem; Preparo do solo; Adubacao, semeadura e cultivares; Entaipamento; Irrigacao; Controle as invasoras; Controle as pragas e molestias; Colheita; Secagem.
650 $arice
650 $aArroz
650 $aOryza Sativa
650 $aPratica Cultural
650 $aProdução
650 $aSistema de Produção
653 $aBrasil
653 $aCultural methods
653 $aProduction
653 $aRio Grande do Sul
653 $aSistema
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